large klenow fragment (New England Biolabs)
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large klenow fragment
Large Klenow Fragment, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 6889 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/large+klenow+fragment/Klenow+Fragment/pm42117222-281-13-16
Average 99 stars, based on 6889 article reviews
Large Klenow Fragment, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 6889 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/large+klenow+fragment/Klenow+Fragment/pm42117222-281-13-16
Average 99 stars, based on 6889 article reviews
large klenow fragment - by Bioz Stars,
2026-09
99/100 stars
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Labeling:Article Title: 3D Chromatin Architecture Provides Insights Into Leaf Trait Variation Among Pear Species. Article Snippet: Subsequently, the cross-linked nuclei were permeated with 0.3% SDS at 65◦C for 10 min. SDS were quenched by 1.8% Triton X100 at 37◦C for 15 min. Chromatin was then digested with 100 U of MboI (NEB) restriction enzyme for 2 h at 37◦C. .. DNA ends were labeled with biotin-14-dCTP (Invitrogen) by incubating with DNA Polymerase I, Article Title: Cooperative Roles of Pds5a and Pds5b Constrain Long‐Range Chromatin Interactions in Vertebrate Embryos Article Snippet: The lysate was centrifuged at 2500 g for 5 min, washed once with ice‐cold Hi‐C lysis buffer, resuspended in 50 μL of 0.5% SDS, and incubated at 62°C for 10 min. After adding 145 μL of water and 25 μL of 10% Triton X‐100, samples were incubated at 37°C for 15 min. Next, 25 μL of 10× NEBuffer 2 and 100 U of MboI were added, and chromatin was digested at 37°C for 2 h with rotation. .. MboI was inactivated by incubation at 62°C for 20 min. DNA ends were labeled with biotin by adding 50 μL of fill‐in master mix (37.5 μL of 0.4 mM biotin‐14‐dATP [Life Technologies], 1.5 μL each of 10 mM dCTP, dGTP, and dTTP, and 8 μL of 5 U/μL DNA Polymerase I Incubation:Article Title: 3D Chromatin Architecture Provides Insights Into Leaf Trait Variation Among Pear Species. Article Snippet: Subsequently, the cross-linked nuclei were permeated with 0.3% SDS at 65◦C for 10 min. SDS were quenched by 1.8% Triton X100 at 37◦C for 15 min. Chromatin was then digested with 100 U of MboI (NEB) restriction enzyme for 2 h at 37◦C. .. DNA ends were labeled with biotin-14-dCTP (Invitrogen) by incubating with DNA Polymerase I, Article Title: Chromatin reorganization drives overexpression of a Btaf1 variant underpinning hematopoietic aging Article Snippet: .. 25 μl of fill-in master mix containing 18.75 μl of 0.4 mM biotin-14-dATP (19524016, ThermoFisher), 0.75 μl of 10 mM dTTP (18255018, ThermoFisher), 0.75 μl of 10 mM dCTP (18253013, ThermoFisher), 0.75 μl of 10 mM dGTP (18254011, ThermoFisher), 4 μl of DNA Polymerase I Article Title: Cooperative Roles of Pds5a and Pds5b Constrain Long‐Range Chromatin Interactions in Vertebrate Embryos Article Snippet: The lysate was centrifuged at 2500 g for 5 min, washed once with ice‐cold Hi‐C lysis buffer, resuspended in 50 μL of 0.5% SDS, and incubated at 62°C for 10 min. After adding 145 μL of water and 25 μL of 10% Triton X‐100, samples were incubated at 37°C for 15 min. Next, 25 μL of 10× NEBuffer 2 and 100 U of MboI were added, and chromatin was digested at 37°C for 2 h with rotation. .. MboI was inactivated by incubation at 62°C for 20 min. DNA ends were labeled with biotin by adding 50 μL of fill‐in master mix (37.5 μL of 0.4 mM biotin‐14‐dATP [Life Technologies], 1.5 μL each of 10 mM dCTP, dGTP, and dTTP, and 8 μL of 5 U/μL DNA Polymerase I DNA Ligation:Article Title: 3D Chromatin Architecture Provides Insights Into Leaf Trait Variation Among Pear Species. Article Snippet: Subsequently, the cross-linked nuclei were permeated with 0.3% SDS at 65◦C for 10 min. SDS were quenched by 1.8% Triton X100 at 37◦C for 15 min. Chromatin was then digested with 100 U of MboI (NEB) restriction enzyme for 2 h at 37◦C. .. DNA ends were labeled with biotin-14-dCTP (Invitrogen) by incubating with DNA Polymerase I, Modification:Article Title: Method of screening for compounds that inhibit proliferation of pancreatic cancer cells having a loss-of-function mutation in the RNF43 gene Article Snippet: .. Briefly, IentiCRISPR pXPR_001 (Addgene 49535) was modified through removal of the sgRNA scaffold region through Ndel/EcoRI digest and blunt ends generation using Purification:Article Title: Influenza A virus NS1 sequesters RNA:DNA hybrids to evade RNase H1-dependent innate immunity Article Snippet: .. Second-strand synthesis was performed on ssDNA using random hexamers, dNTPs, and the Control:Article Title: Functionally-derivatized Nanoantibodies as novel tools for Giardia lamblia cyst detection Article Snippet: .. Control plasmid 139 was generated by excision of the ccdB gene for negative selection in plasmid pBXNPHM3, followed by treatment with DNA Polymerase I Plasmid Preparation:Article Title: Functionally-derivatized Nanoantibodies as novel tools for Giardia lamblia cyst detection Article Snippet: .. Control plasmid 139 was generated by excision of the ccdB gene for negative selection in plasmid pBXNPHM3, followed by treatment with DNA Polymerase I Generated:Article Title: Functionally-derivatized Nanoantibodies as novel tools for Giardia lamblia cyst detection Article Snippet: .. Control plasmid 139 was generated by excision of the ccdB gene for negative selection in plasmid pBXNPHM3, followed by treatment with DNA Polymerase I Selection:Article Title: Functionally-derivatized Nanoantibodies as novel tools for Giardia lamblia cyst detection Article Snippet: .. Control plasmid 139 was generated by excision of the ccdB gene for negative selection in plasmid pBXNPHM3, followed by treatment with DNA Polymerase I Ligation:Article Title: Functionally-derivatized Nanoantibodies as novel tools for Giardia lamblia cyst detection Article Snippet: .. Control plasmid 139 was generated by excision of the ccdB gene for negative selection in plasmid pBXNPHM3, followed by treatment with DNA Polymerase I |